Nanobot synthesisNanobots were prepared as previously described33. In brief, MSNPs were synthesized using a modified Stöber method41, reacting triethanolamine (35 g), ultrapure water (20 ml) and...
Cell linesHuman and murine bladder cancer cells SV-HUC-1, RT4, UM-UC-3, T24, 5637, MB49 were obtained and cultured as previously described . For inducing the...
Animal ethics approvalC57BL/6 mice were obtained from the Fudan University Animal Center.All animals were placed in stainless steel cages in a well-ventilated room maintained...
Animal experimentsFertilized eggs and recipient embryos were provided by local breeders and cPGCs were obtained from 2.5-day-old chicken embryos. The use of these cells...
Human tissueAll experimental protocols were approved by the local ethics committee (Ethik-Kommission der Universität Würzburg, approval number 280/18sc). All experiments were conducted in compliance...
Crypt isolation and organoid cultureCrypt isolation from RCCHan™: WIST female rat duodenum and organoid culture were conducted according to the previous report21. Crypts were...
Acute muscle injury induces Hedgehog activation, via its ligand DHH, to repress IMAT formationThe identity of the endogenous Hh ligand, and its producing cell...
Observations of general indicatorAfter the operation, the mental status of the animal, drinking diet, and fecal traits were typical and healthy.Pathological examination of grossThe...
