Thursday, March 17, 2022 ◉ 1:00 pm EST
Organizer: Dr. Eberhardt Kuhn
Historically, cannabis testing has focused on cannabinoids, i.e. potency. Recently, considerable interest has developed in other constituents of the cannabis plant, like terpenes and flavonoids, as well as undesirable contaminants like pesticides, heavy metals, and mycotoxins. This symposium highlights the latest advances in chromatographic and spectroscopic technologies and method developments to measure these critical compounds.
Presentation 1
Characterization of Terpene Synthase Family Members
Anthony Torres
Cannabis Sativa, a diecious angiosperm, has long history of domestication by humans tracing back to Neolithic times (Ren2021, Tihelka 2021). The plant has been highly sought for its medicinal metabolites and is most well-known for its bioaccumulation of specialized compounds called cannabinoids and terpenoids. Monoterpenes such as Terpinolene, alpha-pinene, and beta myrcene undergo enzymatic synthesis from the precursor Geranyl pyrophosphate (GPP), while sesquiterpenes such as beta caryophyllene, beta farnescene, and alpha bisabolol are synthesized from the precursor farnesyl pyrophosphate (FPP)(Bohlmann 2011). To assess the activity of specific terpene synthase genes among Cannabis varieties in our germplasm, we utilized molecular cloning and functional gene characterization techniques to clone and characterize dominantly expressed terpene synthase genes TPS37 and TPS20…
Original Author Link click here to read complete story..
Thursday, March 17, 2022 ◉ 1:00 pm EST
Organizer: Dr. Eberhardt Kuhn
Historically, cannabis testing has focused on cannabinoids, i.e. potency. Recently, considerable interest has developed in other constituents of the cannabis plant, like terpenes and flavonoids, as well as undesirable contaminants like pesticides, heavy metals, and mycotoxins. This symposium highlights the latest advances in chromatographic and spectroscopic technologies and method developments to measure these critical compounds.
Presentation 1
Characterization of Terpene Synthase Family Members
Anthony Torres
Cannabis Sativa, a diecious angiosperm, has long history of domestication by humans tracing back to Neolithic times (Ren2021, Tihelka 2021). The plant has been highly sought for its medicinal metabolites and is most well-known for its bioaccumulation of specialized compounds called cannabinoids and terpenoids. Monoterpenes such as Terpinolene, alpha-pinene, and beta myrcene undergo enzymatic synthesis from the precursor Geranyl pyrophosphate (GPP), while sesquiterpenes such as beta caryophyllene, beta farnescene, and alpha bisabolol are synthesized from the precursor farnesyl pyrophosphate (FPP)(Bohlmann 2011). To assess the activity of specific terpene synthase genes among Cannabis varieties in our germplasm, we utilized molecular cloning and functional gene characterization techniques to clone and characterize dominantly expressed terpene synthase genes TPS37 and TPS20…
