Primary neuron culture preparationThe experimental protocols involving animal tissue harvesting were approved by the veterinary office of the Canton Basel-Stadt according to Swiss federal...
Ethical statementFor experiments performed at The University of Tokyo, all methods for animal care and use were approved by the institutional review committees of...
Cell cultureBrown adipocytes were differentiated from an immortalized brown preadipocyte mouse cell line kindly provided by B. Spiegelman (Harvard University) as previously described19. Preadipocytes...
Animal experimentsFertilized eggs and recipient embryos were provided by local breeders and cPGCs were obtained from 2.5-day-old chicken embryos. The use of these cells...
Human tissueAll experimental protocols were approved by the local ethics committee (Ethik-Kommission der Universität Würzburg, approval number 280/18sc). All experiments were conducted in compliance...
Crypt isolation and organoid cultureCrypt isolation from RCCHan™: WIST female rat duodenum and organoid culture were conducted according to the previous report21. Crypts were...
Mouse modelsWild type and transgenic animals used to generate X-MET were housed in a temperature-controlled (22 °C) room with a 12-h light/dark cycle. All animal...
EthicsThis study was conducted following the principles of the Helsinki Declaration. The use of human iPSCs was approved by the ethics committee at Keio...
