Cell cultureBrown adipocytes were differentiated from an immortalized brown preadipocyte mouse cell line kindly provided by B. Spiegelman (Harvard University) as previously described19. Preadipocytes...
MiceYoung (2–4 months old) and old (18–26 months old) male and female wild-type C57BL6/J, Lgr5-ki-eGFP-creER, and Olfm4-ki-eGFP-creER mice were group housed and maintained in...
Ethics statementAll iPSC lines were previously derived from skin biopsy fibroblasts, following signed informed consent, collected under ethical approval granted by the South Wales...
Crypt isolation and organoid cultureCrypt isolation from RCCHan™: WIST female rat duodenum and organoid culture were conducted according to the previous report21. Crypts were...
As the summer heat rolls in, what better way to cool down and unwind than with refreshing cannabis-infused beverages? Cannabis infusions offer a unique...
MaterialsTLR7/8 agonist 1 dihydrochloride was purchased from Cayman Chemical. 1,2-Epoxydodecane (C12) was obtained from Sigma-Aldrich. Core 200 was customized from Enamine, and other polyamine...
Mouse modelsWild type and transgenic animals used to generate X-MET were housed in a temperature-controlled (22 °C) room with a 12-h light/dark cycle. All animal...
Cell culture and cell linesHEK293T cells (Pricella, CL-0005) were cultured on 0.12% gelatin pre-coated dishes with DMEM high glucose (HyClone, SH30243.01) supplemented with 10%...
