Fluorescence in situ hybridization (FISH) is the gold standard for visualizing genomic DNA in fixed cells and tissues, but it is incompatible with live-cell imaging, and its combination with RNA imaging is challenging. Consequently, due to its capacity to bind double-stranded DNA (dsDNA) and design flexibility, the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (CRISPR-Cas9) technology has sparked enormous interest over the past decade. In this review, we describe various nucleic acid (NA)- and protein-based (amplified) signal generation methods that achieve imaging of repetitive and single-copy sequences, and even single-nucleotide variants (SNVs), next to highly multiplexed as well as dynamic imaging in live cells.
Indianapolis, Indiana – The Plaintiff, Oakley, Inc. (“Oakley”), is an American company operating as an independent subsidiary of Luxottica Group S.p.A. Oakley designs, develops...
Ukraine’s recent two-front counteroffensive has dealt a heavy blow to the Russian military. Contrary to Western military orthodoxy, air superiority was not a prerequisite...
The U.S. Patent and Trademark Office issued the following 203 patent registrations to businesses and individuals in Indiana during August 2022, based on applications...
According to Statista, the number of mobile AR users is forecasted to grow to 1.73 billion by 2024. The rise of e-commerce has paved the way for many retail businesses…
New research paper titled “Semiconducting silicon–phosphorus frameworks for caging exotic polycations” from researchers at Department of Chemistry, Iowa State University, and Ames Laboratory, U.S....
