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Tag: PCR

Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity – npj Vaccines

EthicsAll animal experimental procedures were approved by the Chonnam National University Institutional Animal Care and Use Committee under the protocol CNU IACUC-H-2021-51. Animal research...

Rapid, tunable, and multiplexed detection of RNA using convective array PCR – Communications Biology

Integrating reverse transcription in caPCRWe first introduced an RT step to the standard caPCR assay. caPCR is performed using a set of 4 heaters...

C9orf72-ALS human iPSC microglia are pro-inflammatory and toxic to co-cultured motor neurons via MMP9 – Nature Communications

Ethics statementAll iPSC lines were previously derived from skin biopsy fibroblasts, following signed informed consent, collected under ethical approval granted by the South Wales...

mRNA vaccine quality analysis using RNA sequencing – Nature Communications

Design and synthesis of reference plasmidA reference construct was first designed, with the intention of optimising the production of RNA therapeutics for pre-clinical research....

Phenotypic screening of signaling motifs that efficiently induce cell proliferation – Scientific Reports

Construction of plasmidsThe whole amino acid sequence of each constructed receptor is shown in Supplementary Information (Supplementary Fig. 1). A backbone plasmid pMK-stuffer-IPTG25 was used...

Derivation of transgene-free bat induced pluripotent stem cells amenable to chimera formation in mice, pigs, and chicks – Cell Discovery

Dear Editor,The unique biological characteristics of bats, including longevity, antiviral properties, hibernation, and echolocation, have garnered significant attention from the scientific community. To enhance...

Haspin balances the ratio of asymmetric cell division through Wnt5a and regulates cell fate decisions in mouse embryonic stem cells – Cell Death Discovery

Cell cultureE14 mESCs were purchased from the Chinese Academy of Sciences Stem Cell Bank. They were cultured at 37 °C in 5% CO2 in an...

A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering – Communications Biology

Nielsen, J., Larsson, C., van Maris, A. & Pronk, J. Metabolic engineering of yeast for production of fuels and chemicals. Curr. Opin. Biotechnol. 24,...

Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment – Cell Death & Disease

Cell linesHuman and murine bladder cancer cells SV-HUC-1, RT4, UM-UC-3, T24, 5637, MB49 were obtained and cultured as previously described . For inducing the...

Adipose-derived mesenchymal stem cell therapy for reverse bleomycin-induced experimental pulmonary fibrosis – Scientific Reports

Animal ethics approvalC57BL/6 mice were obtained from the Fudan University Animal Center.All animals were placed in stainless steel cages in a well-ventilated room maintained...

In vitro transcription of self-assembling DNA nanoparticles – Scientific Reports

Gene-bearing DNA origami scaffolds were prepared as previously described24, summarized as follows.Standard PCRPrimers for amplification of the Green Fluorescent Protein (GFP) gene were designed...

Single-cell analysis of lizard blastema fibroblasts reveals phagocyte-dependent activation of Hedgehog-responsive chondrogenesis – Nature Communications

Heterogeneous fibroblasts contribute to lizard tail regrowthTo investigate the complexity and heterogeneity of each regeneration state during tail regrowth, we performed single-cell RNA sequencing...

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